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hamster mab anti mouse cd3 pevio770  (Miltenyi Biotec)


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    Miltenyi Biotec hamster mab anti mouse cd3 pevio770
    Hamster Mab Anti Mouse Cd3 Pevio770, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1227 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hamster+anti+cd3/CD3%CE%B5+Antibody%2C+anti-mouse/pmc12390538-90-5-9
    Average 94 stars, based on 1227 article reviews
    hamster mab anti mouse cd3 pevio770 - by Bioz Stars, 2026-10
    94/100 stars

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    Incubation:

    Article Title: SRC2 controls CD4 T cell activation via stimulating c-Myc-mediated up- regulation of amino acid transporter Slc7a5
    Article Snippet: Suspensions of 5 × 105 cells/ml in RPMI-1640 medium (Corning Inc) containing 2 mM Lglutamine, 50 μM β-mercaptoethanol, 100 U/ml penicillin, 100 mg/ml streptomycin and 10% FBS (Corning Inc) were cultured in 24-well or 48-well plates pre-coated with 0.1 mg/ml rabbit antihamster. .. The medium was supplemented with 0.25 μg/ml hamster anti-CD3 and 0.5 μg/ml hamster anti-CD28, and followed with incubation with polarizing cytokines: 2 ng/ml TGF-β (Miltenyi Biotec), 20 ng/ml IL-6 (Miltenyi Biotec) and 20 ng/ml IL-23 (Miltenyi Biotec), or 2 ng/ml TGFβ (Miltenyi Biotec) and 20 ng/ml IL-6 (Miltenyi Biotec), or 20 ng/ml IL-1β (Miltenyi Biotec), 20 ng/ml IL-6 (Miltenyi Biotec) and 20 ng/ml IL-23 (Miltenyi Biotec) for Th17 differentiation; and 20 ng/ml IL-12 (Miltenyi Biotec) for Th1 differentiation. ..



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    Malaria-responsive T cells migrate to target organs. (A) Schematic representation of experimental design. Donor animals were infected 5 days before adoptive transfer. T cells from the donors’ spleens were enriched in wool columns and transferred to naive acceptor animals, generating two groups: acceptors that received T cells from uninfected donors (Naive → Naive); acceptors that received T cells from infected donors (Infected → Naive). On the third day after adoptive transfer, T cell migration was analyzed, and renal function was assessed. (B) Parasitemia was observed only in donor infected mice (gray bar) (n = 6–8). (C) Migration of malaria-responsive T cells to the kidney by <t>CD3</t> + /CFSE + cells (n = 4). (D) To confirm migration to the kidney, C57BL/6-GFP animals, infected or not, were used as donors to perform adoptive transfer (n = 6). Migration is expressed as the percentage of CD3 + /CFSE + or CD3 + /GFP + cells relative to the total CD3 + cells in the organ of interest. *versus naive → naive group, P < 0.05.
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    Image Search Results


    Malaria-responsive T cells migrate to target organs. (A) Schematic representation of experimental design. Donor animals were infected 5 days before adoptive transfer. T cells from the donors’ spleens were enriched in wool columns and transferred to naive acceptor animals, generating two groups: acceptors that received T cells from uninfected donors (Naive → Naive); acceptors that received T cells from infected donors (Infected → Naive). On the third day after adoptive transfer, T cell migration was analyzed, and renal function was assessed. (B) Parasitemia was observed only in donor infected mice (gray bar) (n = 6–8). (C) Migration of malaria-responsive T cells to the kidney by CD3 + /CFSE + cells (n = 4). (D) To confirm migration to the kidney, C57BL/6-GFP animals, infected or not, were used as donors to perform adoptive transfer (n = 6). Migration is expressed as the percentage of CD3 + /CFSE + or CD3 + /GFP + cells relative to the total CD3 + cells in the organ of interest. *versus naive → naive group, P < 0.05.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: CD8 + T cells promote tubule-interstitial damage in malaria-induced acute kidney injury

    doi: 10.3389/fcimb.2025.1561806

    Figure Lengend Snippet: Malaria-responsive T cells migrate to target organs. (A) Schematic representation of experimental design. Donor animals were infected 5 days before adoptive transfer. T cells from the donors’ spleens were enriched in wool columns and transferred to naive acceptor animals, generating two groups: acceptors that received T cells from uninfected donors (Naive → Naive); acceptors that received T cells from infected donors (Infected → Naive). On the third day after adoptive transfer, T cell migration was analyzed, and renal function was assessed. (B) Parasitemia was observed only in donor infected mice (gray bar) (n = 6–8). (C) Migration of malaria-responsive T cells to the kidney by CD3 + /CFSE + cells (n = 4). (D) To confirm migration to the kidney, C57BL/6-GFP animals, infected or not, were used as donors to perform adoptive transfer (n = 6). Migration is expressed as the percentage of CD3 + /CFSE + or CD3 + /GFP + cells relative to the total CD3 + cells in the organ of interest. *versus naive → naive group, P < 0.05.

    Article Snippet: For T cell migration experiments, the cell suspension was incubated with a PeCy5.5-conjugated hamster IgG1 anti-murine CD3 antibody (145-2C11, eBioscience, San Diego, CA, USA).

    Techniques: Infection, Adoptive Transfer Assay, Migration